Intracytoplasmic Sperm Injection (ICSI) Micropipettes
 ICSI
Micropipettes are available either angled or straight in configuration.
The shape and taper of these needles are unique in the industry,
and as a result provide for quicker movement of fluids and
spermatozoa.
These needles are made from borosilicate glass stock
which is thoroughly washed with Milli-Q 18M ultrapure water
and sterilized by dry heat before tooling. Finished needles are
individually inspected for perfection of tip size and micro-spike
dimensions under 400X magnification before packaging in sterile,
toxicity tested holders.
Holding Micropipettes
Holding Micropipettes may be ordered in two standard size ranges
in either straight or angled configuration, depending on preference.
(Custom orders available.)
Assisted Hatching Micropipettes
Assisted Hatching Micropipettes are available in a standard bore
diameter of 10-15 µm, and can be ordered in either the straight
or angled configuration. They are designed for precise dissolving
of the zona pellucida and can be ordered in conjunction with acidic
Tyrodes media formulated specifically for this purpose.
Denuding Micropipettes
D enuding
Micropipettes are available in five popular sizes and arrive 4
per package, sterile and ready-to-use. Depending on the size ordered,
these pipettes are used for denudation of coronal cells to evaluate
fertilization status following conventional insemination, or clearance
of these cells following hyaluronidase treatment prior to ICSI.
The larger sizes are used to safely manipulate fertilized oocytes
or embryos for a variety of purposes.
Hyaluronidase Preparation
This formulation is designed for the safe removal of coronal cells
adhering to the zona pellucida. All components have been cell culture
tested prior to sale. This ready-to-use mixture consists of Hyaluronidase
(80u/ml) in HEPES-buffered HTF, supplemented with albumin. It is
stable for 60 days at 4-8°C, and longer if kept frozen.
Polyvinylpyrrolidone (PVP) Solution
This product is used to slow sperm movement as well as coat the
inner bore of ICSI Micropipettes allowing for smooth transfer of
a spermatozoon into the ooplasm. PVP is dissolved into the HTF-HEPES
Plus Albumin and is filtered through 0.8µm nitrocellulose
membranes prior to packaging. It is stable at 4-8°C for up
to 60 days.
Acidified Medium
This media is used for local dissolving of the zona pellucida
when used with assisted hatching micropipettes. It is available
ready-to-use with a pH rangeof 2.3 - 2.5 and is stable for up to
60 days at 4-8°C. The polyvinylpyrrolidone (PVP) used in the
AT mixture has been culture tested.
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