• Intracytoplasmic Sperm Injection (ICSI) Micropipettes
• Holding Micropipettes
• Assisted Hatching Micropipettes
• Denuding MicroPipettes
• Hyaluronidase Preparation
• Polyvinylpyrrolidone (PVP) Solution
• Acidified Medium

ICSI Micropipettes are available either angled or straight in configuration. The shape and taper of these needles are unique in the industry, and  as a result provide for quicker movement of fluids and spermatozoa. These needles are made from borosilicate glass stock which is thoroughly washed with Milli-Q 18M  ultrapure water and sterilized by dry heat before tooling. Finished needles are individually inspected for perfection of tip size and micro-spike dimensions under 400X magnification before packaging in sterile, toxicity tested holders. 

Holding Micropipettes 

Holding Micropipettes may be ordered in two standard size ranges in either straight or angled configuration, depending on preference. (Custom orders available.) 

Assisted Hatching Micropipettes

Assisted Hatching Micropipettes are available in a standard bore diameter of 10-15 µm, and can be ordered in either the straight or angled configuration. They are designed for precise dissolving of the zona pellucida and can be ordered in conjunction with acidic Tyrodes media formulated specifically for this purpose.

Denuding Micropipettes 

Denuding Micropipettes are available in five popular sizes and arrive 4 per package, sterile and ready-to-use. Depending on the size ordered, these pipettes are used for denudation of coronal cells to evaluate fertilization status following conventional insemination, or clearance of these cells following hyaluronidase treatment prior to ICSI. The larger sizes are used to safely manipulate fertilized oocytes or embryos for a variety of purposes.

Hyaluronidase Preparation 

This formulation is designed for the safe removal of coronal cells adhering to the zona pellucida. All components have been cell culture tested prior to sale. This ready-to-use mixture consists of Hyaluronidase (80u/ml) in HEPES-buffered HTF, supplemented with albumin. It is stable for 60 days at 4-8°C, and longer if kept frozen. 

Polyvinylpyrrolidone (PVP) Solution 

This product is used to slow sperm movement as well as coat the inner bore of ICSI Micropipettes allowing for smooth transfer of a spermatozoon into the ooplasm. PVP is dissolved into the HTF-HEPES Plus Albumin and is filtered through 0.8µm nitrocellulose membranes prior to packaging. It is stable at 4-8°C for up to 60 days.

Acidified Medium

This media is used for local dissolving of the zona pellucida when used with assisted hatching micropipettes. It is available ready-to-use with a pH rangeof 2.3 - 2.5 and is stable for up to 60 days at 4-8°C. The polyvinylpyrrolidone (PVP) used in the AT mixture has been culture tested.